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KMID : 0380019940090040378
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 4 p.378 ~ p.383
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Selection of High Yielding Mutant Strains for the Antifungal Antibiotics KRF-001
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이항우/Lee, Hang Woo
김무경/김성욱/손광희/복성해/Kim, Moo Kyung/Kim, Sung Uk/Son, Kwang hee/Bok, Song Hae
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Abstract
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An improved method for the selective isolation of high-yielding mutant strains for the production of antifungal antibiotic KRF-001 was investigated. The mutant strain U. V 4, which produces high titer of KRF-001, was selected on the high potency agar plate after ultraviolet light irradiation. The I1. V 4 strain produced 2-fold more KRF-001 than the mother strain in production media. Large scale fermentation was performed using the U. V 4 strain in 100k fermenter. The antifungal antibiotic KRF-001 secreted into culture broth was detected by HPLC in 24hrs of fermentation.
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